RNA-Seq of Chloridea virescens: adult female antennae - SRA

SRX3510908: RNA-Seq of Chloridea virescens: adult female antennae
1 ILLUMINA (Illumina HiSeq 2500) run: 47M spots, 11.8G bases, 6.3Gb downloads

Design: Adult female Chloridea virescens were sacrificed by freezing at -80C. Antennae were subsequently removed from a single female just prior to RNA isolation. 2 antennae (one pair) were used for extraction. RNA was isolated according to Trizol protocol, then processed on RNEasy micro columns for clean up. RNA was quantified using Bioanalyzer and Qubit, the sent to the NY Genome Center for library preparation and sequencing. Libraries were prepared using the Ovation RNA-Seq System for preparing amplified cDNA from total RNA followed by Illumina TruSeq Nano DNA Sample Preparation. 125bp paired-end sequencing was performed on an Illumina HiSeq 2500.

Submitted by: American Museum of Natural History

Study: Antennal and thoracic RNA-Seq data from Chloridea spp. moths. Raw sequence reads

PRJNA423106 • SRP127519 • All experiments • All runs

show Abstracthide Abstract

We examined the transcriptomes of antennal and thoracic tissue from two closely related Chloridea (Lepidoptera:Noctuidae:Heliothinae) species. Chloridea virescens is a broad generalist pest of economic importance, while Chloridea subflexa is a narrow specialist on plants in the genus Physalis. To identify genes that are expressed in male and/or female antennae from one or both species but not in non-sensory tissues, we sequenced male and female antennae from each species, and from combined male+female thoracic tissue from each species.

Sample: CvirFant

SAMN08211370 • SRS2788340 • All experiments • All runs

Organism: Heliothis virescens

Library:

Name: CvirFant

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Runs: 1 run, 47M spots, 11.8G bases, 6.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR6417880	47,011,200	11.8G	6.3Gb	2019-01-01

ID:: 4879123

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity